The importance of disinfection in dentistry is always emphasized due to the serious risk of cross-contamination among the patient, clinician, and lab personnel. It has become immensely significant, especially after the COVID-19 pandemic. However, the disinfection material and process can induce changes in the physical properties of various materials used in record-making. Hence this investigation was undertaken to assess the hardness and dimensional stability of three interocclusal record materials after disinfection with iodophor. Materials and methods: Polyvinyl siloxane, Aluwax, and Zinc Oxide Eugenol (ZOE) impression paste were the materials used for this study. The material was mixed and put into a stainless steel die. The materials were divided into 3 groups with 2 subgroups having 10 samples each. The samples were subjected to immersion in 0.5% iodophor for 10 minutes. Dimensional changes and surface hardness of the samples were experimented with by traveling microscope and hardness tester respectively. Results: Polyvinylsiloxane (PVS) (Jet Bite) showed the least change in terms of dimensional stability and surface hardness when immersed in 0.5% iodophor for 10 minutes. Conclusion: Polyvinylsiloxane (Jet Bite) was found to be having considerable dimensional stability and surface hardness before and after disinfection, which was heeded by ZOE paste and Aluwax respectively.