Statement of Problem The adhesion of impression material to impression tray is very important. Tray adhesive plays a major role in making accurate impression. Although manufactures recommend the use of particular tray adhesives, comparison of their affective adhesiveness has not been reported. The effect of use of tray adhesives on dimensional accuracy of dies has not been established. Purpose The aim of this study was to compare the dimensional accuracy of dies using different tray adhesives at different time intervals. Materials and Methods First part of study comprised 120 samples in six groups with 20 samples in each group. First group comprised samples with no tray adhesive, and in other five groups, different types of tray adhesives were applied. The dies obtained were evaluated for upper diameter, lower diameter, and occlusogingival height. In the second part, there were a total of 125 samples in five groups with 25 samples in each group. Five different types of tray adhesive were applied for five different time intervals 5, 10, 15, 20, and 25 minutes, respectively. The specimens were tested in tensile mode for its debonding force at a crosshead speed of 5 mm/min, until separation failure occurred. Results Significant difference was seen for upper and lower diameters when compared with the group without any tray adhesive. The maximum bond strength was found in the group in which tray adhesive was applied for 20 minutes.