Citation Information :
Singh H, Bishen KA, Garg D, Sukhija H, Sharma D, Tomar U. Fixation and Fixatives: Roles and Functions—A Short Review. 2019; 7 (2):51-55.
Fixation is considered as physiochemical process where cells or tissues are fixed chemically. Fixatives perform various functions such as prevention of autolysis and tissue putrefaction. Various fixative agents include formaldehyde, glutaraldehyde, osmium tetroxide, glyoxal, picric acid, and so on. A detailed search on PubMed, Google scholar, and Scopus database showed very few articles on “fixation” and “fixative.” Keeping this fact in mind, a comprehensive review on fixation and fixatives was prepared. The main aim of this review is to make pathologists and laboratory technicians familiar with the basic aspects and different types of fixatives.
Histotechnology: A Self Instructional Text. 3rd ed. American Society for Clinical Pathology Press, Hong Kong
Demonstration of substances of innate immunity in the esophagus epithelium of domesticated mammals. Part I—methods and comparative fixation evaluation. Acta Histochem 2011;113(2):175–188
The use of electronic cell volume analysis with the AMAC II to determine the optimum glutaraldehyde fixative concentration for nucleated mammalian cells. Scan Electron Microsc 1978;2:1123
Histopathology. 2nd ed. London: Oxford University Press; 2018
Histomorphologic assessment of formalin substitute fixatives for diagnostic surgical pathology. Arch Pathol Lab Med 2005;129(4):502–506
The reactions of formaldehyde with amino acids and proteins. Adv Pro Cherlz 1945;2:277–335
Formaldehyde as a probe of DNA structure. I. Reaction with exocyclic amino groups of DNA bases. Biochemistry 1975;14(6):1281–1296
Formaldehyde as a probe of DNA structure. II. Reaction with endocyclic imino groups of DNA bases. Biochemistry 1975;14(6):1297–1303
Reaction mechanisms of formaldehyde with endocyclic imino groups of nucleic acid bases. J Am Chem Soc 1994;116(8):3548–3555
3rd ed. American Chemical Society Monograph Series. New York NY: Reinhold Publishing; 1964
5th ed. Butterworth-Heinemann; 1976
Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation. J Cell Biol 1963;17:19–58
Some aspects of fixation with glutaraldehyde. A biochemical and histochemical comparison of the effects of formaldehyde and glutaraldehyde fixation on various enzymes and glycogen, with a note on penetration of glutaraldehyde into liver. J Anat 1967;101(Pt 1):83–92
Introduction to the theory and practice of fixation of tissues. J Histotechnol 2001;24(3):173–190
Cell and tissue fixation, 1972–1 982. Histochern J 1985;17(4):389–442
Fixatives and fixation: a review. Histochem J 1969;1(4):323–360
Biological Applications. 2nd ed, Vol. 1: Baltimore, MD: University Park Press; 1981
A comparison of microwaves and heat alone in the preparation of tissue for electron microscopy. Histochem J 1990;22(6-7):358–364
New York, NY: Academic Press; 1981
Princeton, NJ: Princeton University Press; 1998
Fixation with mercury salts. Acta Histochem Suppl 1973;13(0):107–118
Glyoxal, a non-irritating aldehyde suggested as substitute for formalin in histological fixations. Science 1943;982539: 204
Übergang antimikrobieller Wirkstoffe von der Fläche in die Luft. Hyg Med 1984;9:259–260
Henry's law constants of some environmentally important aldehydes. Environ Sci Technol 1988;22(12):1415–1418
Advanced concepts in fixation: 1. Effects of fixation on immunohistochemistry, reversibility of fixation and recovery of proteins, nucleic acids, and other molecules from fixed and processed tissues. 2. Developmental methods of fixation. J Histotechnol 2001;24(3):201–210
, Glyoxal: A Technical Brochure. Wayne, NJ: American Cyanamid Company; 1986;50
5th ed. New York, NY: Oxford University Press; 1980
Gaithersburg, MD: American Histolabs; 1992
In: Woods AE, Ellis RC, eds. Laboratory Histopathology. New York, NY: Churchill Livingstone; 1994
4th ed. New York, NY: McGraw-Hill; 1976
1st ed. Melbourne, Australia: Termarcarphi; 1981
Pol André Bouin, MD (1870-1962). Bouin's fixative and other contributions to medicine. Arch Pathol Lab Med 1992;116(8):882–884
Immunohistochemical assays in prostatic biopsies processed in Bouin's fixative. J Clin Pathol 2005;58(3):322–324
In: Damjanov I, Linder J, eds. Anderson's Pathology. 10th ed. Maryland Heights, MO: Mosby; 1996
The use of acrolein as a fixative for light and electron microscopy. Anat Rec 1959;133:305
Acrolein—a fine structure fixative for viral cytochemistry. J Cell Biol 1963;18(1):207–213
Stability of a biological tissue fixed with a naturally occurring crosslinking agent (genipin) J Biomed Mater Res 2001;55(4):538–546
In vitro evaluation of cytotoxicity of a naturally occurring cross-linking reagent for biological tissue fixation. J Biomater Sci Polym Ed 1999;10(1):63–78
In vitro evaluation of cytotoxicity of diepoxy compounds used for biomaterial modification. J Biomed Mater Res 1995;29(7):829–834
Fixation of biological tissues with a naturally occurring crosslinking agent: fixation rate and effects of pH, temperature, and initial fixative concentration. J Biomed Mater Res 2000;52(1):77–87
Drummond JL, Yamauchi M. Application of crosslinkers to dentin collagen enhances the ultimate tensile strength. J Biomed Mater Res B Appl Biomater 2007;80(1):268–272
Genipin-cross-linked collagen/chitosan biomimetic scaffolds for articular cartilage tissue engineering applications. J Biomed Mater Res A 2010;95(2):465–475
Genipin-crosslinked chitosan/gelatin blends for biomedical applications. J Mater Sci Mater Med 2008;19(2):889–898
